Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers

In this study, Lactobacillus reuteri B2 was isolated from the feces of C57BL/6 mice and assessed on probiotic activity. L. identified by 16S rDNA sequencing, which cell viability in acidic conditions at pH 2.0 64% after 2 h, presents 0.30% bile salts, 6 37%. Antimicrobial assay with showed maximum diameters against Klebsiela oxytoca J7 (12.5 ± 0.71 mm). We further hypothesized if strain free form can survive all gastrointestinal tract (GIT) then utilization appropriate biomaterials would ameliorate its stability GIT. microencapsulated into sodium alginate-(Na-alg) different content Na-alg maleate (SM) beads. Characterization materials enveloped their thermal characteristics (TGA/DTA analysis) structure using: scanning electron microscopy (SEM), FTIR, particle size distribution. The high survival rate low to 4.0 presence concentrations up 0.30%, obtained. strong antimicrobial activity best protection + SM simulated gastric juices (SGJ).